We narrowed to 50,410 results for: ANG

1. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

   Plasmid

   #213526

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed H424 to R

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

   Plasmid

   #221896

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TH0994-pOCC177-Fus-wt_(1-211)

   Plasmid

   #221886

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   PLD domain (aa 1-211)

   Promoter

   PH promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

   Plasmid

   #221890

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   HNRNPA3 (HNRNPA3 Human)
   

   Use

   Tags

   6xHis-MBP-PreScission-mGFP-TEV

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-Myc-PSK2 (1-416) (K57A)

   Plasmid

   #197124

   Purpose

   Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

   Insert

   TAOK1 (aa1-416, K57A) (TAOK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Changed K 57 to A and deleted C-terminus

   Promoter

   SV40

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2-UbC-3xNLS-mScarlet-I

   Plasmid

   #191099

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet-I
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   optimized to human codon usage

   Promoter

   UbC

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY315

   Plasmid

   #184160

   Purpose

   Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-zntA-1034, PrhaB, and Ptet

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLY162

   Plasmid

   #184138

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R1, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY163

   Plasmid

   #184141

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R2, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY168

    Plasmid

    #184142

    Purpose

    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-Ar2, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY300

    Plasmid

    #184158

    Purpose

    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-oxyS-86, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY320

    Plasmid

    #184161

    Purpose

    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

    Plasmid

    #128666

    Purpose

    mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Flag-Polß(TM-K206A)-IRES-puro

    Plasmid

    #128679

    Purpose

    mammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R/K206A

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Flag-Polß(TM-KTM)-IRES-puro

    Plasmid

    #128676

    Purpose

    mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R/-K41R/K61R/K81R

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-Flag-Polß(KTM)-IRES-Neo

    Plasmid

    #128670

    Purpose

    mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K41R/K61R/K81R

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

    Plasmid

    #128657

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-Flag-Polß(TM-KTM)-IRES-neo

    Plasmid

    #128675

    Purpose

    mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R-K41R/K61R/K81R

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-Flag-Polß(TM-K244A)-IRES-puro

    Plasmid

    #128680

    Purpose

    mammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R/K244A

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge