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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML RING

Plasmid

#185631

Purpose

expression of GST-tagged PML RING domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML RB1

Plasmid

#185636

Purpose

expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1302-AAV-EFSNC-dCjCas9-KRAB

Plasmid

#223147

Purpose

Expression of KRAB with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1(W795R)-StayGold

Plasmid

#210822

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Mutation

Changed Trp-795 to Arg

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-TwinStrep-His

Plasmid

#210816

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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95.a_pSTC0-zeo

Plasmid

#202334

Purpose

Design 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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95.b_pSTC0-zeo

Plasmid

#202335

Purpose

Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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90.a_pSTC0-zeo

Plasmid

#202336

Purpose

Design 90.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

90.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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90.b_pSTC0-zeo

Plasmid

#202337

Purpose

Design 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

90.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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80.a_pSTC0-zeo

Plasmid

#202338

Purpose

Design 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

80.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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80.b_pSTC0-zeo

Plasmid

#202339

Purpose

Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

80.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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70.a_pSTC0-zeo

Plasmid

#202340

Purpose

Design 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

70.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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70.b_pSTC0-zeo

Plasmid

#202341

Purpose

Design 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

70.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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50.a_pSTC0-zeo

Plasmid

#202342

Purpose

Design 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

50.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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50.b_pSTC0-zeo

Plasmid

#202343

Purpose

Design 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

50.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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rw-consensus_pSTC0-zeo

Plasmid

#202349

Purpose

Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

rw-consensus

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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98.a_pSTC0-zeo

Plasmid

#202332

Purpose

Design 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge