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Hy_pMT dati MCS circfirefly_500

Plasmid

#110114

Purpose

Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-pre-let-7_PShairpin

Plasmid

#26537

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

pre-let-7_PS hairpin

Use

Retroviral

Tags

Expression

Mammalian

Mutation

5 nt mutated to make pre-let-7 stem pairing perfe…

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
ND4 DdCBE G1

Plasmid

#221978

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE C2

Plasmid

#221979

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T1

Plasmid

#221980

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T2

Plasmid

#221981

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 alphaDdCBE G1

Plasmid

#221982

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 alphaDdCBE C2

Plasmid

#221983

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 alphaDdCBE T1

Plasmid

#221984

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 alphaDdCBE T2

Plasmid

#221985

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GFP E17TAG/Q157TAG

Plasmid

#68299

Purpose

E17/Q157 TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP E17TAG/Q157TAG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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E17TAG GFP zeo resistance

Plasmid

#69118

Purpose

For E17TAG GFP reporter expression in non-zeocin resistant strains

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Use

Tags

His

Expression

Bacterial

Mutation

E17 to TAG

Promoter

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only
pChlr-7SE

Plasmid

#52168

Purpose

Encodes module 7 with amino acids 12S-16E for recognition of nt 2G

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 241-283 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-4SYE

Plasmid

#52156

Purpose

Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

D43951 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pChlr-3CQ

Plasmid

#52147

Purpose

Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 97-132 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-1SQ

Plasmid

#52139

Purpose

Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 25-60 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-2NQ

Plasmid

#52145

Purpose

Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 61-96 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-4NQ

Plasmid

#52153

Purpose

Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 133-168 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pChlr-5CQ

Plasmid

#52159

Purpose

Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 169-204 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge