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We narrowed to 4,504 results for: pcr

Showing: 1051 - 1080 of 4504 results

  1. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  2. pOpen-THEAQlig

    Plasmid
    #165535
    Purpose
    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Taq DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165535-plasmid-map-sequence-id-339125

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  3. XE18 Noggin-CSKA

    Plasmid
    #16998
    Depositor
    Randall Moon
    Insert
    noggin PCR clone (nog.L Frog)
    Use
    Xenopus expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2008
    Availability
    Academic Institutions and Nonprofits only

  4. pRS414-Syf1 817 Truncation

    Plasmid
    #234126
    Purpose
    Syf1 PCR truncation starting at 817 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234126-plasmid-map-sequence-id-466534

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  5. pRS414-Syf1 634 Truncation

    Plasmid
    #234116
    Purpose
    Syf1 PCR truncation starting at 634 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234116-plasmid-map-sequence-id-466457

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  6. pRS414-Syf1 778 Truncation

    Plasmid
    #234115
    Purpose
    Syf1 PCR truncation starting at 778 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234115-plasmid-map-sequence-id-465993

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  7. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

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  8. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

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  9. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    P45
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  10. Lox-Stop-Lox TOPO Hygro

    Plasmid
    #35688
    Depositor
    Jacqueline Lees
    Insert
    loxP-STOP-loxP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2012
    Availability
    Academic Institutions and Nonprofits only
    35688-plasmid-map-sequence-id-43895

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  11. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  12. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

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  13. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

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  14. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

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  15. pFA6a-SRM (sortase recognition motif)-hphMX4

    Plasmid
    #54488
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    hphMX4
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

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  17. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

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  18. pTargetAmp

    Plasmid
    #228552
    Purpose
    Template for PCR amplification for gRNA cloning plasmid by in vivo cloning.
    Depositor
    Jeferson Gross
    Insert
    Ampicillin resistance
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228552-plasmid-map-sequence-id-454861

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  19. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid
    #54489
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    kanMX6
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  21. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  22. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  23. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  24. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  25. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  26. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  28. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

    Plasmid
    #219695
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG2-ER-tagBFP-tUBQ10-GG3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219695-plasmid-map-sequence-id-435727

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  29. L0I75_tUBQ10_GG5-GG6

    Plasmid
    #219714
    Purpose
    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG5-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT terminator with added golden gate linkers
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219714-plasmid-map-sequence-id-439236

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  30. L0-T13 GG3-tRBCS-mCherry-NLS-GG4

    Plasmid
    #219696
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG3-tRBCS-mCherry-NLS-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219696-plasmid-map-sequence-id-435728

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Showing: 1051 - 1080 of 4504 results