-
Plasmid#121757PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsert3x HA epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JA001: pMVP (L3-L2) FLAG epitope tag + WPRE
Plasmid#121758PurposepMVP L3-L2 entry plasmid, contains FLAG epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertFLAG epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IZ901: pMVP (L3-L2) OLLAS epitope tag + WPRE
Plasmid#121759PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertOLLAS epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IZ702: pMVP (L3-L2) myc epitope tag + WPRE
Plasmid#121760PurposepMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertmyc epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE
Plasmid#121761PurposepMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsert6x His epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH201: pMVP (L5-L4) 3x HA epitope tag
Plasmid#121700PurposepMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert3x HA epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG703: pMVP (L5-L4) 6x His epitope tag
Plasmid#121706PurposepMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert6x His epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO801: pMVP (L5-L4) 3x FLAG epitope tag
Plasmid#121702PurposepMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert3x FLAG epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2+IA
Plasmid#111418Purposeexpress murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2+IA
Plasmid#111273Purposeexpress murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-CMV-Cer-FL- Bmal L95E
Plasmid#47374PurposeBmal full length with cer tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenusExpressionMammalianMutationL95EPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA/BmalF423R
Plasmid#47348PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationF423RPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
RBMS2_2xRRM_pGEX
Plasmid#135132PurposeRecombinant expression of RBMS2 with dual-affinity tagDepositorInsertRBMS2 (RBMS2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
mCherry KNL1 wt hardened
Plasmid#45224DepositorInsertmCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
UseTagsTEV S15ExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP KNL1 wt hardened
Plasmid#45223DepositorInsertGFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)
UseTagsTEV S15ExpressionMutationE172GPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kindlin2
Plasmid#105305PurposeTo study effect of Kindlin 2 on Cell/ECM adhesionDepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ7
Plasmid#223180PurposepORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.DepositorTypeEmpty backboneUseChromosomal integrationTagsExpressionMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK036 desmaMCS:minCerulean,cryaa:Venus
Plasmid#195981PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorUseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-rM1PYK
Plasmid#220232PurposeInducible expression of rabbit muscle pyruvate kinase isoform 1DepositorInsertRabbit muscle pyruvate kinase (PKM )
UseTags6xHis-MBP-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only