-
-
GST-CHMP4A (205-222) (pGEX2T(TEV))
Plasmid#80633PurposeResidues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222;PromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3.1-Zeo_BACE1
Plasmid#227105PurposeBACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assaysDepositorInsertBACE1 (BACE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pCI-MS2V5-eRF3a F76a
Plasmid#65811Purposeexpression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1DepositorInserteRF3a (GSPT1 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationF76aPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp12
Plasmid#165117Purposemammalian expression and localizationDepositorInsertNsp12 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
TFORF3124
Plasmid#144600PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry KNL1 wt hardened and RVSF->AAAA
Plasmid#45226DepositorInsertmCherry KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)
UseTagsTEV S15ExpressionMutationRVSF 58 AAAAPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorInsertNbVHH05-HA-His (UBE2J1 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mScarlet-hCenpC
Plasmid#171490PurposeT7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNADepositorInsertCenpC (CENPC Human)
UsePgehmeTagsmScarletExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3 H194L
Plasmid#122689PurposeExpresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationChanged Histidine 194 to LeucinePromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3 S368A
Plasmid#122680PurposeExpresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationchanged Serine 368 to AlaninePromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0113
Plasmid#141520PurposeLentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP KNL1 wt hardened and RVSF->AAAA
Plasmid#45225DepositorInsertGFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)
UseTagsExpressionMutationRVSF mutated to AAAAPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0114
Plasmid#141521PurposeLentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Leucyl tRNA synthetase pRK5
Plasmid#46341DepositorInsertleucyl tRNA synthetase (LARS1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0115
Plasmid#141522PurposeLentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
