We narrowed to 1,336 results for: cbs

1. pCDNA3-HA/Bmal L95E

   Plasmid

   #47344

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

2. LA601: pMVP (L3-L2) P2A-Puro + WPRE

   Plasmid

   #121785

   Purpose

   pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-Puro + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NOVA1_Full-length_pGEX

   Plasmid

   #135147

   Purpose

   Recombinant expression of NOVA1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   NOVA1 (NOVA1 Human)
   

   Tags

   GST-SBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. KZ501: pMVP (L3-L2) P2A-Puro + polyA

   Plasmid

   #121780

   Purpose

   pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-Puro + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

   Plasmid

   #121830

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/KRAB (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KG802: pMVP (L3-L2) V5 epitope tag + polyA

   Plasmid

   #121755

   Purpose

   pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   V5 epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PCBP4_3xKH_pGEX

   Plasmid

   #135156

   Purpose

   Recombinant expression of PCBP4 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP4 (PCBP4 Human)
   

   Tags

   GST-SBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LA001: pMVP (L3-L2) P2A-mCherry + WPRE

   Plasmid

   #121774

   Purpose

   pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-mCherry + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. KA501: pMVP (L3-L2) pA; CMV::TETa-pA

   Plasmid

   #121801

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG69 G6PD 3'UTR mut

    Plasmid

    #12053

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    G6PD 3'UTR mut (G6PD Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV10/3xflag-Clock L57E/L113E/W284A

    Plasmid

    #47343

    Purpose

    triple mutation used for co-IP

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E/L113E/W284A

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAG150 FGFR1 3'UTR

    Plasmid

    #12008

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR (FGFR1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

    Plasmid

    #121828

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

    Plasmid

    #121762

    Purpose

    pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    V5 epitope tag + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3-HA/Bmal I317D

    Plasmid

    #47347

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    I317D

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3-HA/Bmal L115E

    Plasmid

    #47345

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid

    #121812

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC-mTET1-N

    Plasmid

    #81056

    Purpose

    bacterial expression of N-terminus of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    N-terminus, amino acids 301-1366

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. LA102: pMVP (L3-L2) P2A-eYFP + WPRE

    Plasmid

    #121778

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eYFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

    Plasmid

    #121839

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge