Addgene

We narrowed to 12,635 results for: sequence

Showing: 701 - 720 of 12635 results

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Use

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Mutation

Promoter

pMT

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pG108 - K

Plasmid

#178039

Purpose

Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Insert

Kanamycin resistance

Use

Tags

Expression

Bacterial

Mutation

ErmAM has been replaced with Kanamycin for select…

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBCm

Plasmid

#46763

Purpose

E.coli genomic integration at arsB

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-beta2-adrenergic receptor

Plasmid

#66994

Purpose

Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-beta2-adrenergic receptor (ADRB2 Human)

Use

Tags

Human Growth hormone Signal Sequence-HaloTag

Expression

Mammalian

Mutation

The signal sequence from human growth hormone was…

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-IVT

Plasmid

#215834

Purpose

Template for in vitro transcription of CBE6b (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRL278

Plasmid

#70256

Purpose

sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

Depositor

Peter Wolk

Article

Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

Type

Empty backbone

Use

Conditionally lethal

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAcGFP-MALAT1M

Plasmid

#120382

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

MALAT1 (MALAT1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6b

Plasmid

#215830

Purpose

Express CBE6b (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-eNme2C-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6c

Plasmid

#215831

Purpose

Express CBE6c (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-eNme2C-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

AAVS1-specific sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-XNS

Plasmid

#196655

Purpose

Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GAVPO

Plasmid

#159923

Purpose

GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

GAVPO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Lac

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviQIX (NDel15)

Plasmid

#198357

Purpose

Express M.CviQIX (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviQIX

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-At-TRL

Plasmid

#32242

Depositor

Jay Hesselberth, Stan Fields

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Use

Tags

6xHistidine

Expression

Bacterial

Mutation

Promoter

T7 RNA Polmyerase

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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