We narrowed to 17,211 results for: EMB

Showing: 7181 - 7200 of 17211 results

Results per page:

Sort results by:

pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
UBQ10:sXVE:S10-(MCS)

Plasmid

#108177

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBQ10-moxMaple-GW

Plasmid

#209353

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Stx1a-12aa-mCer

Plasmid

#53236

Purpose

Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

Syntaxin 1A (Stx1a Rat)

Tags

12aa-linker and mCerulean

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL-p27UTR

Plasmid

#20881

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p27 3'UTR (Cdkn1b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pMK453 (BSD-P2A-dTAG-mCherry)

Plasmid

#214383

Purpose

dTAG-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-dTAG-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCC25

Plasmid

#177319

Purpose

Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

Depositor

Rafal Tomecki

Article

Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

Insert

TtCutA 240-603

Tags

6xHis-SUMOTag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE100

Plasmid

#59395

Purpose

MethyloBrick cloning vector, no promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

none

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LL - hNANOGi

Plasmid

#12196

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

NANOG - RNAi (NANOG Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
SB_loop

Plasmid

#110103

Purpose

pBXPHM3 with scaffold Sybody of the loop library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the loop library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-mCerulean3-pBluescript

Plasmid

#166757

Purpose

For purification of mCerulean3 fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge