We narrowed to 18,079 results for: comp

1. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199363

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSP3-FR-2Strep

   Plasmid

   #210315

   Purpose

   Expresses SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 3xFLAG-SENP6

   Plasmid

   #42887

   Depositor

   Edward Yeh

   Article

   Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

   Insert

   SENP6 (SENP6 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO GFP HSPA1L

   Plasmid

   #19484

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA1L (HSPA1L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SAE1

   Plasmid

   #175241

   Purpose

   Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SAE1 (SAE1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SAE2-mGarnet

   Plasmid

   #175240

   Purpose

   Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBA2 (UBA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ER-ABKAR

   Plasmid

   #61508

   Purpose

   Expression of AMPK biosensor targeted to the endoplasmic reticulum

   Depositor

   Takanari Inoue, Jin Zhang

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Use

   Tags

   ER targeting sequence from CYP450 2C1 (see commen…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAd-Track Flag-SIRT1

   Plasmid

   #8438

   Depositor

   Pere Puigserver

   Article

   Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC_N_CCDC93

    Plasmid

    #209687

    Purpose

    Expresses MAC-tagged CCDC93 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

    Insert

    CCDC93 (CCDC93 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CFAST-Halotag-ER

    Plasmid

    #214413

    Purpose

    ER localization of CFAST fused to Halotag-ER

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Use

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNHA-C1-HseIF4A3_I

    Plasmid

    #146578

    Purpose

    Mammalian Expression of HseIF4A3

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HseIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. myc-hIFITM3-delta59-68

    Plasmid

    #104361

    Purpose

    Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deleted amino acids 59 through 68

    Promoter

    CMV

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. wt dynamin 1 pEGFP

    Plasmid

    #34680

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5/FRT/TO DNAJB5

    Plasmid

    #19473

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB5 (DNAJB5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS human c-Myc

    Plasmid

    #14971

    Depositor

    Joan Massague

    Article

    Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

    Insert

    c-Myc (MYC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GW1-rogRFP2

    Plasmid

    #207876

    Purpose

    Mammalian expression of the red fluorescent rogRFP2 redox sensor

    Depositor

    Mathew Tantama

    Article

    Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

    Insert

    rogRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid

    #129383

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

    Depositor

    Silvestro Conticello

    Article

    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

    Insert

    mCherry-ApoB-Tag-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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