We narrowed to 16,131 results for: form

1. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1α and pTRE3G

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-MT mouse Axin (Axin MTFu1)

   Plasmid

   #21287

   Depositor

   Frank Costantini

   Article

   Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

   Insert

   Axin (Axin1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-puro-mycRSK2wt

   Plasmid

   #15827

   Depositor

   Jing Chen

   Article

   Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

   Insert

   Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 LC3A WT

   Plasmid

   #123069

   Purpose

   Gateway entry clone encoding human MAP1LC3A wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-EGFP-P2A-FusionRed

   Plasmid

   #191108

   Purpose

   Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   pAAV-CAG-EGFP-P2A-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mSin1 shRNA #1

   Plasmid

   #13483

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1 (MAPKAP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

7. LLP773_pLenti-6xHRBKET-sgRNA

   Plasmid

   #211766

   Purpose

   Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
   

   Use

   Lentiviral

   Promoter

   pCMV and U6

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP778_Traptavidin-p65HSF1-CO

   Plasmid

   #211771

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-p65HSF1
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP776_SpyCatcher-p65HSF1-CO

   Plasmid

   #211769

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-p65HSF1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP786_SpyCatcher-EZH2-FL-CO

    Plasmid

    #211779

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-KRAB
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag Jnk2a2(apf)

    Plasmid

    #13761

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2(apf) (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif THr(183)-Pro-T…

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAX-mScarlet-CIP4

    Plasmid

    #178364

    Purpose

    Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Tags

    GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mSin1 shRNA #2

    Plasmid

    #13484

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mSin1 (MAPKAP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-Ms-c-Myc-W136E

    Plasmid

    #26024

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    W136E

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

17. F40 tension sensor

    Plasmid

    #101252

    Purpose

    Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-F40-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sema6d.4-AP-His

    Plasmid

    #72044

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IRES-Blast coSHMT2

    Plasmid

    #106299

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Codon optimized

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-IP-EGFP-mAtg5

    Plasmid

    #38196

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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