We narrowed to 13,228 results for: sequence
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Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-tTA
Plasmid#84502PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV2-MESA-35F-M-tTA
UseAAVTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pV1F
Plasmid#205084PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialPromoteragorbacterium virB operon promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)
Plasmid#232127PurposeA middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOZC-PALB2
Plasmid#133552PurposePALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.DepositorInsertPALB2 (PALB2 Human)
UseRetroviralAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAluYNF1
Plasmid#50933PurposeExpresses dimeric AluY RNA in mammalian cells.DepositorInsertAluYNF1
ExpressionMammalianPromoter7SL RNA promoter (pol III)Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtBCP1Pro
Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-terminal Halo-XLF
Plasmid#207546PurposeHomologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
TagsHaloTagExpressionMammalianAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR-Kras
Plasmid#123357PurposeFRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.DepositorInsertAktAR-Kras
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-tTA
Plasmid#84500PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV1-MESA-45F-M-tTA
UseAAVTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-Ctrl
Plasmid#102355PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-1.3
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only