We narrowed to 7,672 results for: CCH

1. pRL_GI_PCB28

   Plasmid

   #98745

   Purpose

   For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

   Inserts

   heme oxygenase 1 (TED4 Mustard Weed)
   Phycocyanobilin:ferredoxin oxidoreductase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   ADH1m and PGK1

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAI-GAL4-CREB-S133A

   Plasmid

   #46770

   Purpose

   Expression of GAL4 fusion to CREB-S133A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
   

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 133 to Alanine

   Promoter

   CMV

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNAI-GAL4-CREB-S142A

   Plasmid

   #46771

   Purpose

   Expression of GAL4 fusion to CREB-S142A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
   

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 142 to Alanine

   Promoter

   CMV

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 1 kb prom. + Enhancer CD274

   Plasmid

   #107005

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   N.B. There are two published SNPs in this region …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Trim46

   Plasmid

   #176402

   Purpose

   TRIM46 tagged with GFP

   Depositor

   Casper Hoogenraad, Thomas Schwarz

   Article

   van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

   Insert

   trim46 (Trim46 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Trim46

   Plasmid

   #176401

   Purpose

   TRIM46 tagged with mCherry

   Depositor

   Casper Hoogenraad, Thomas Schwarz

   Article

   van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

   Insert

   trim46 (Trim46 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-1_6xHis-TEV-mCh-NDP52

   Plasmid

   #187829

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Tags

   6xHisTag, TEV, and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

   Plasmid

   #107009

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo-RL-ZEB1 200bmutx5

    Plasmid

    #35537

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    5 mutations in miR200b seed regions of 3'UTR…

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-EGFR*-CUbo

    Plasmid

    #212821

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

    Plasmid

    #107001

    Purpose

    Expresses epitope-tagged double-mutant murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Changed Ser52 to Ala and Ser178 to Ala

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL His-p97

    Plasmid

    #113510

    Purpose

    Expression of His-p97 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97 (VCP Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLD-puro-Cn-VA-NHEJ1-WT

    Plasmid

    #141339

    Purpose

    Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    NHEJ1 (NHEJ1 Human)
    

    Use

    Lentiviral

    Tags

    VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. kanMX6-ins2

    Plasmid

    #195039

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLINC-AKAR1-CAAX

    Plasmid

    #87705

    Purpose

    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FLINC-AKAR1-CAAX
    

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC-SUR-YR

    Plasmid

    #61768

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    Magnaporthe oryzae ILV promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. natMX6-ins5

    Plasmid

    #195042

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105983

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    SYFP2-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Promoter

    human Synapsin 1

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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