We narrowed to 9,520 results for: try

1. JE301: pMVP (L5-L4) Tir1-P2A-AID

   Plasmid

   #121722

   Purpose

   pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1-P2A-AID (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc (C-term on osTIR1)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-PGK-hKLF4

   Plasmid

   #60435

   Purpose

   Over-expression of human KLF4

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   KLF4 (KLF4 Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-TEV-3XFLAG

   Plasmid

   #105784

   Purpose

   Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-3XFLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265F2

   Plasmid

   #164722

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)NG-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBacI-KDAC8 D101E

   Plasmid

   #224350

   Purpose

   Expresses human KDAC8 (HDAC8) D101E in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Aspartate 101 mutated to glutamate

   Promoter

   Polyhedrin

   Available Since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. STK24_HUMAN_D0

   Plasmid

   #79742

   Purpose

   This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK24 (STK24 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

   Plasmid

   #141236

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   NES-jRGECO1a
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   6xHIS tag and nuclear export signal

   Promoter

   human elongation factor-1 alpha (EF-1 alpha)

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TT 3xFLAG-KPNA2

   Plasmid

   #192306

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KPNA2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNA2 (KPNA2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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