We narrowed to 17,014 results for: EMB

1. pET26_MraYaa

   Plasmid

   #100166

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Chung et al Science. 2013 Aug 30;341(6149):1012-1016

   Insert

   mraY (phospho-MurNAc-pentapeptide translocase)
   

   Tags

   MBP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Col1a-3'probe

   Plasmid

   #20731

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   Col1a-3'probe (Col1a1 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-NFLAG TRF1 deltaA

   Plasmid

   #16063

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   Deleted acidic domain (aa1-66)

   Available Since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pRU53

   Plasmid

   #167679

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBI-eGFP-Rab31

   Plasmid

   #200265

   Purpose

   Expression of eGFP-Rab31 (human) in mammalian cells

   Depositor

   Guangpu Li

   Article

   Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

   Insert

   Rab31 (human cDNA) (RAB31 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc-hIFITM3

   Plasmid

   #58461

   Purpose

   Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pFP.R265

   Plasmid

   #138167

   Purpose

   Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(VDA).M86.(SDL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE101

   Plasmid

   #59396

   Purpose

   MethyloBrick cloning vector, no promoter; KmR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-Z22

   Plasmid

   #120065

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SNAP
   

   Use

   Pcr template

   Tags

   SNAP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptRNA100

    Plasmid

    #154133

    Purpose

    Expresses a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    tRNAs operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGUS (GB0208)

    Plasmid

    #68203

    Purpose

    Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    GUS coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-TAZ-N-Flag

    Plasmid

    #19025

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

13. Integrin beta1(1-455)-tev-BHCys

    Plasmid

    #27315

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin beta1 (ITGB1 Human)
    

    Expression

    Mammalian

    Mutation

    beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

14. FRB-ECFP(W66A)-Giantin

    Plasmid

    #67903

    Purpose

    Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    FRB-ECFP(W66A)-Giantin
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU54

    Plasmid

    #167680

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. GLI-3 bs-2

    Plasmid

    #16420

    Depositor

    Bert Vogelstein

    Article

    Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

    Insert

    GLI3 (GLI3 Human)
    

    Expression

    Bacterial

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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