We narrowed to 15,515 results for: LEA

1. pmCherry-N1-EXO1b-D173A

   Plasmid

   #111622

   Purpose

   Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b D173A (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIPZ-bioGEF-SUMO2nc-PURO

   Plasmid

   #208043

   Purpose

   Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   SUMO2nc (SUMO2 Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Q90P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218914

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GFP-EphrinB3-Flag(A227Y)

   Plasmid

   #155013

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   A227Y

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLRC1-NEK10p:NEK10-3XFLAG

   Plasmid

   #137030

   Purpose

   Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

   Depositor

   David Sabatini

   Article

   Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

   Insert

   NIMA-like kinase 10 (NEK10 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Promoter

   human NEK10 endogenous promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218910

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4155C>T and 4729T>G and 4731…

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-DIO-GRAB_CRFmut

   Plasmid

   #208663

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

   Plasmid

   #208960

   Purpose

   A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

   Plasmid

   #208953

   Purpose

   A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-HRV3C

    Plasmid

    #164567

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike - Furin site changed for HRV3C site
    

    Use

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    682-685 (furin site) replaced with HRV3C site (LE…

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

    Plasmid

    #185446

    Purpose

    Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-BG505.SOSIP-QES.i03.c01-TM

    Plasmid

    #111845

    Purpose

    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    CD5 leader sequence and TM helix from MHC class I

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-mOrange2-NEDD1-gTBD

    Plasmid

    #196858

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD (NEDD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROF346

    Plasmid

    #155331

    Purpose

    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mGLDC

    Plasmid

    #72881

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    Gldc (Gldc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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