We narrowed to 18,937 results for: Tec

1. ABE8e(TadA-8e K20A R21A)

   Plasmid

   #138496

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e K20A R21A)-nSpCas9
   

   Expression

   Mammalian

   Mutation

   TadA-8e K20A R21A

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CABE-T2.6

   Plasmid

   #193263

   Purpose

   Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.6
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CBE-T1.6

   Plasmid

   #193275

   Purpose

   Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.6
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC94

   Plasmid

   #118912

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNAS1b split mCherry NEDD4-2 WW2

   Plasmid

   #111883

   Purpose

   Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Tags

   Split mCherry system: NEDD4-2 WW2 fused to C-term…

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMal-SLFN2

   Plasmid

   #174322

   Purpose

   Bacterial expression of MBP-tagged mouse SLFN2

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   SLFN2 (Slfn2 Mouse)
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM4889 (pCV0001)

   Plasmid

   #120079

   Purpose

   Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
   

   Use

   Other

   Mutation

   mobA Y25F

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVsc.U6-sgFah.CMV/CB-EGFP

   Plasmid

   #121508

   Purpose

   Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2238

    Plasmid

    #36689

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD7-TALEN_Left (Chd7 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2239

    Plasmid

    #36690

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD7-TALEN_Right (Chd7 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pOCC254

    Plasmid

    #118889

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; mCherry, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid

    #181747

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEos2-Talin-IBS2-7

    Plasmid

    #57424

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin IBS2 (Tln1 Mouse)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Talin Integrin Binding Site

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRSETa-Amrose v1

    Plasmid

    #79537

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 1

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. UASgal-A9-Pleum

    Plasmid

    #60343

    Purpose

    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCfB3053(gRNA X-2, XI-5, XII-4)

    Plasmid

    #73295

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Zebrafish-gRNA-0001

    Plasmid

    #42241

    Purpose

    gRNA targeted to zebrafish gene apoea

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-apoea (apoea Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only