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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R124A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-TIAL1

Plasmid

#207669

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

Depositor

Christine Mayr

Publication

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TIAL1 (TIAL1 Human)

Tags

MCP-mCherry

Expression

Mammalian

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Publication

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHLP700

Plasmid

#203742

Purpose

C-terminal tagging of target proteins with AID*/3xHA degron

Depositor

Mark Hall

Publication

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain codons 71-114

Use

Pcr amplification template for gene tagging

Tags

3xHA

Promoter

none

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTY137 psfGFP-bimax2

Plasmid

#202410

Purpose

Expresses sfGFP-bimax2 to inhibit importin-alpha-mediated nuclear import

Depositor

Tim Mitchison

Publication

Yoo et al J Cell Biol. 2021 Jul 5;220(7). pii: 212

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bimax2

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1978

Plasmid

#144319

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TBX1 (TBX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-OptoPB

Plasmid

#113935

Purpose

Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

Depositor

Yubin Zhou

Publication

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OptoPB

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1a#2/Cre

Plasmid

#173572

Purpose

Expresses a Arid1a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Publication

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1a (Arid1a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_KanR neo

Plasmid

#167877

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Publication

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MANF-Halo

Plasmid

#175341

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MANF (MANF Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pT2MP.EF-pHlu

Plasmid

#129304

Purpose

pH measurement, Tol2 transposon

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pHluorin (from H. Imamura)

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVd1-EGFP-hRhoA-wt

Plasmid

#133926

Purpose

Low level expression of GFP fused human RhoA wild type

Depositor

Frank Bradke, Sebastián Dupraz

Publication

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-hRhoA WT (RHOA Human)

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-TTc-4xDQNAT

Plasmid

#128401

Purpose

Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate5

Plasmid

#164999

Purpose

Expression of candidate TCR #5 TCRalpha cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMVTCRa_candidate10

Plasmid

#165007

Purpose

Expression of candidate TCR #10 TCRalpha cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate5

Plasmid

#165000

Purpose

Expression of candidate TCR #5 TCRbeta cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGBW-m4133269

Plasmid

#152855

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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