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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,181 results for: nes

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Showing: 7261 - 7280 of 16181 results

  1. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  5. KIFC3

    Plasmid
    #25537
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIFC3 (KIFC3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    ATPase domain only
    Available since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. AA061

    Plasmid
    #216018
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-NG (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216018-plasmid-map-sequence-id-431502

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  7. AA029

    Plasmid
    #216009
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-NG (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216009-plasmid-map-sequence-id-431424

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  8. cr3Dd

    Plasmid
    #184862
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system
    Depositor
    Patrick Sobetzko
    Publication
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184862-plasmid-map-sequence-id-366226

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  9. EGFP-CCDC32

    Plasmid
    #110505
    Purpose
    Fluorescent CCDC32
    Depositor
    Bernardo Mainou
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCDC32 (CCDC32 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110505-plasmid-map-sequence-id-210723

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  10. mCherry-CCDC32

    Plasmid
    #110506
    Purpose
    Fluorescent CCDC32
    Depositor
    Bernardo Mainou
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCDC32 (CCDC32 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110506-plasmid-map-sequence-id-210721

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  12. AA031

    Plasmid
    #216011
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-SpRY (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216011-plasmid-map-sequence-id-431442

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  13. AA030

    Plasmid
    #216010
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-SpG (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216010-plasmid-map-sequence-id-431432

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  15. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  17. mCherry-PLEKHS1

    Plasmid
    #110510
    Purpose
    Fluorescent PLEKHS1
    Depositor
    Bernardo Mainou
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLEKHS1 (C10orf81 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110510-plasmid-map-sequence-id-210717

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  19. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  20. JEH127

    Plasmid
    #179299
    Purpose
    Expresses dCas9 fused to VPR
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    CAG
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179299-plasmid-map-sequence-id-354392

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Showing: 7261 - 7280 of 16181 results