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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GWF180

Plasmid

#133607

Purpose

CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

DNCR2-VPR, NS3aH1-tagBFP-SpdCas9

Expression

Mammalian

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pJBL704

Plasmid

#140374

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-4BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-595

Plasmid

#11634

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 1.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

Plasmid

#192380

Purpose

To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-CYLD

Plasmid

#27547

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Available Since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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