We narrowed to 18,079 results for: comp

1. pSmart-EF1a-Nla-cas7

   Plasmid

   #178879

   Purpose

   Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas7c-bGH PolyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

   Plasmid

   #199348

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1 Utr261-EGFP

   Plasmid

   #58471

   Purpose

   Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Utr261 (UTRN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1 gfaABC1D-Glt1-eGFP

   Plasmid

   #176862

   Purpose

   Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-hAtg13

   Plasmid

   #31965

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   Atg13 (ATG13 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pFRT/FLAG/HA-DEST QKI

   Plasmid

   #19891

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-Skp2

   Plasmid

   #19946

   Depositor

   Yue Xiong

   Article

   Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

   Insert

   S-phase kinase-associated protein 2 (p45) (SKP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET16b-Trx CC32/35SS

   Plasmid

   #36946

   Depositor

   Anna Blom

   Article

   King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

   Insert

   Thioredoxin 1 (TXN Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   both active site cysteines (32,35) mutated to ser…

   Promoter

   T7

   Available since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pSLC-255

   Plasmid

   #124292

   Purpose

   Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

   Depositor

   Swaine Chen

   Article

   Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

   Insert

   vsfGFP-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-NavMs

    Plasmid

    #100004

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

    Depositor

    Paul DeCaen

    Article

    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

    Insert

    His-NavMs, Voltage gated sodium channel
    

    Use

    Tags

    Hexa-His tag followed by a thrombin cleavage site

    Expression

    Mammalian

    Mutation

    C-terminus from a codon-optimized synthetic gene,…

    Promoter

    CMV

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. xCas9(3.6)-BE4

    Plasmid

    #108386

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

    Plasmid

    #184394

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS-HIV-1 Group O-vpr

    Plasmid

    #115836

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group O Vpr (L20571)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-hUVRAG

    Plasmid

    #24296

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NFAST(low)-mApple-6xHp

    Plasmid

    #214412

    Purpose

    Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-6xHp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pISH-Gap43

    Plasmid

    #74336

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

    Insert

    growth associated protein 43 (Gap43 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFLAG-CMV14-mULK1

    Plasmid

    #24301

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. opto-Tiam1

    Plasmid

    #174508

    Purpose

    Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Tiam1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. psiCHECK2-miR-34 WT

    Plasmid

    #78258

    Purpose

    psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    fully complimentary miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-neo-HA-hAtg14

    Plasmid

    #24294

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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