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35S-ZFN-pDGB3alpha1

Plasmid

#240729

Purpose

Encodes a ZFN that targets the broken GUS:NPTII reporter.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

ZFN

Expression

Plant

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.1

Plasmid

#238542

Purpose

Golden gate acceptor backbone for position 1 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.2

Plasmid

#238543

Purpose

Golden gate acceptor backbone for position 2 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.3

Plasmid

#238544

Purpose

Golden gate acceptor backbone for position 3 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Gal4-LATeNT(slow)

Plasmid

#240985

Purpose

Expresses Gal4-LATeNT* cargo in mammalian cells; used with UAS-mCherry reporter

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

HA-GAL4-VAMP2(2-94)-V5-LATeNT

Tags

HA and V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCherry-LATeNT

Plasmid

#240989

Purpose

Expresses mCherry-LATeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-mCherry-LATeNT

Plasmid

#240991

Purpose

Flp-dependent expression of LATeNT

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LATeNT(slow)-Arrestin

Plasmid

#240984

Purpose

Expresses LATeNT*-Arrestin in mammalian cells; used with CCR6-NanoLuc or KORD-NanoLuc

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

LATeNT*-myc-Arrestin

Tags

myc (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_cpCys203

Plasmid

#247771

Purpose

bacterial expression of cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus and His6-TEV

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisM5RT

Plasmid

#216672

Purpose

Expresses his-tagged Mut5 reverse transcriptase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

thermostable M-MuLV reverse transcriptase variants

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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yEPAC-R279L pDRF1-GW

Plasmid

#246454

Purpose

Expresses the non-responsive yEPAC-R279L sensor in budding yeast.

Depositor

Bas Teusink

Article

Botman et al Mol Biol Cell. 2021 Jun 15;32(13):1229-1

Insert

yEPAC-R269L

Expression

Yeast

Promoter

PMA1

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1983 UbL40-mCherry

Plasmid

#242440

Purpose

Ae aegypti UbL40 promoter expressing mCherry

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

mCherry

Expression

Insect

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

Plasmid

#241843

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

VP16

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

VP16 minimal activation domain x 3

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST6GalNAc2 WT

Plasmid

#239827

Purpose

Displays Fc-fusion hST6GalNAc2 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST6GalNAc2 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-62 of hST6GalNAc2 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-pST3Gal1 WT

Plasmid

#239822

Purpose

Displays Fc-fusion pST3Gal1 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-pST3Gal1 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(CD94)-Fc-pST3Gal1 WT

Plasmid

#239821

Purpose

Displays Fc-fusion pST3Gal1 WT on mammalian cell surface via transmembrane domain of CD94

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(CD94)-Fc-pST3Gal1 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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