164,809 results

1. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yomRuby2-Kan

   Plasmid

   #44953

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. KillerRed-LacI-NLS

   Plasmid

   #215102

   Purpose

   Expresses KillerRed-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   KillerRed-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PH-PLC-delta-EGFP

   Plasmid

   #136998

   Purpose

   Lentiviral EGFP lipid sensor for PI(4,5)P2

   Depositor

   Ken-Ichi Takemaru

   Insert

   PH-PLC-delta-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-T7gene1

   Plasmid

   #165505

   Purpose

   Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEBTet_NADP-Snifit-cyto

   Plasmid

   #113917

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis-SNAP-PI(3,4,5)P3 Probe

   Plasmid

   #211514

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BTK1-PH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry Vinculin

    Plasmid

    #50527

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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