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pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)

Plasmid

#160645

Purpose

Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos

Use

Synthetic Biology

Expression

Plant

Mutation

BsmBI sites removed

Promoter

35S x2

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2E

Plasmid

#224441

Purpose

Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQGYQ insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3D

Plasmid

#224445

Purpose

Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYREL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3E

Plasmid

#224446

Purpose

Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4B

Plasmid

#224449

Purpose

Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV4D

Plasmid

#224451

Purpose

Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV4C

Plasmid

#224450

Purpose

Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSM insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV3C

Plasmid

#224444

Purpose

Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV3B

Plasmid

#224443

Purpose

Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001162231)

Plasmid

#76409

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001149414)

Plasmid

#75502

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SRC gRNA (BRDN0001145402)

Plasmid

#75650

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRC (SRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001149025)

Plasmid

#76395

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SRC gRNA (BRDN0001149457)

Plasmid

#75652

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRC (SRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FXN gRNA (BRDN0001144784)

Plasmid

#77813

Purpose

3rd generation lentiviral gRNA plasmid targeting human FXN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FXN (FXN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FXN gRNA (BRDN0001145648)

Plasmid

#77814

Purpose

3rd generation lentiviral gRNA plasmid targeting human FXN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FXN (FXN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FXN gRNA (BRDN0001146360)

Plasmid

#77815

Purpose

3rd generation lentiviral gRNA plasmid targeting human FXN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FXN (FXN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SRC gRNA (BRDN0001146185)

Plasmid

#75651

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRC (SRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

Plasmid

#210710

Purpose

This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/N580A

Promoter

M11

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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DGKQ gRNA (BRDN0001162275)

Plasmid

#77377

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKQ (DGKQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K3 gRNA (BRDN0001148097)

Plasmid

#76016

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K3 (MAP3K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AXL gRNA (BRDN0001146797)

Plasmid

#76700

Purpose

3rd generation lentiviral gRNA plasmid targeting human AXL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AXL (AXL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LRPPRC gRNA (BRDN0001145987)

Plasmid

#77773

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRPPRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRPPRC (LRPPRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AXL gRNA (BRDN0001147657)

Plasmid

#76701

Purpose

3rd generation lentiviral gRNA plasmid targeting human AXL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AXL (AXL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK16 gRNA (BRDN0001147125)

Plasmid

#76903

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK16 (CDK16 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AXL gRNA (BRDN0001146919)

Plasmid

#76702

Purpose

3rd generation lentiviral gRNA plasmid targeting human AXL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AXL (AXL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK16 gRNA (BRDN0001146694)

Plasmid

#76901

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK16 (CDK16 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK16 gRNA (BRDN0001147123)

Plasmid

#76902

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK16 (CDK16 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge