We narrowed to 17,014 results for: EMB

1. TAL-L1-1.3-Ctrl

   Plasmid

   #102355

   Purpose

   Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-1.3
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   FLAG and HA

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBJZ_001

   Plasmid

   #155357

   Purpose

   Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJBJZ_006

   Plasmid

   #155359

   Purpose

   Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMK451 (BSD-P2A-dTAG)

   Plasmid

   #214381

   Purpose

   dTAG for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-dTAG
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

   Plasmid

   #202719

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Gdf3

   Plasmid

   #13358

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   growth differentiation factor 3 (Gdf3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MinC

   Plasmid

   #133628

   Purpose

   Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

   Insert

   His-MinC (minC E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-C1V1-TT-mRuby2-ST

   Plasmid

   #109124

   Purpose

   Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   C1V1-TT-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R272

    Plasmid

    #138221

    Purpose

    Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Myo 1g

    Plasmid

    #134991

    Purpose

    Expression of mouse Myo 1g in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Olety et al FEBS Lett. 2010 Feb 5;584(3):493-9. doi:

    Insert

    Myo 1g (Myo1g Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE:CloverCP-EFS:ZeoR

    Plasmid

    #68478

    Purpose

    Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    CloverCP
    Zeocin Resistance
    

    Use

    Lentiviral

    Tags

    "CP" degradation domain

    Expression

    Mammalian

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-FKBP-SAC1C389S

    Plasmid

    #108128

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSC236

    Plasmid

    #216368

    Purpose

    xhNup155-Nb2i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb2i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSC235

    Plasmid

    #216372

    Purpose

    xhNup155-Nb3i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb3i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSC227

    Plasmid

    #216351

    Purpose

    xhNup35-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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