We narrowed to 17,014 results for: EMB

1. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE103

   Plasmid

   #59398

   Purpose

   MethyloBrick expression vector, fumC promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PfumC

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-HA-GlpG

   Plasmid

   #155135

   Purpose

   Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK162_mCherry-4.1G-hardened

   Plasmid

   #46361

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G (EPB41L2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Modified for siRNA resistance to gcTccTcaTttCgaAc…

   Promoter

   CMV

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-Z28

   Plasmid

   #120071

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xHA
   

   Use

   Pcr template

   Tags

   3xHA

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL-L1-1.3-VP64

   Plasmid

   #103033

   Purpose

   Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-1.3-VP64
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   HA and VP64

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BSP188

   Plasmid

   #110917

   Purpose

   For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   unc54 Terminator
   

   Use

   Synthetic Biology; Yeast homologous recombination…

   Expression

   Worm

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-flag-mKdm4d(H189A)-polyA

   Plasmid

   #61554

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (catalytic mutant)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   changed Histidine 189 (of Kdm4d) to Alanine

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMP4518

   Plasmid

   #107778

   Purpose

   Expression of EYFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EYFP in pBBR1-mcs5
   

   Tags

   pLacZ-EYFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXNubWtgate

    Plasmid

    #105080

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    NubI

    Expression

    Yeast

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. BcLOV-mCh-EGFR

    Plasmid

    #208274

    Purpose

    Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    BcLOV-mCherry-EGFR
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    pCMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDH42(CUG/A)

    Plasmid

    #154132

    Purpose

    Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    engineered bacterial rRNAs operon under control of pL promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBFP (GB0025)

    Plasmid

    #68193

    Purpose

    Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    BFP coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL-L1-2.4-VP64

    Plasmid

    #103039

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-2.4-VP64
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    HA and VP64

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Promoter

    longCaMV35S

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDendra2-H2a

    Plasmid

    #157816

    Purpose

    Fluorescent reporter for histone H2A tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H2A (H2ac13 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE104

    Plasmid

    #59399

    Purpose

    MethyloBrick expression vector, coxB promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PcoxB

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-ChETA-mRuby2-ST

    Plasmid

    #109126

    Purpose

    Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChETA-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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