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pDONR223_CHD2_p.E412K

Plasmid

#81469

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway entry vector

Mutation

E412K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_YTHDC1_WT

Plasmid

#81799

Purpose

Gateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YTHDC1 (YTHDC1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRS1_WT

Plasmid

#82214

Purpose

Gateway Donor vector containing IRS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRS1 (IRS1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.P124S

Plasmid

#81703

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

P124S; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

Plasmid

#154430

Purpose

hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

Depositor

David Liu

Article

Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

Insert

hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

Use

AAV and CRISPR

Expression

Mammalian

Mutation

VRQR point mutations in SpCas9

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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R713-M88-303: CMV51p> Halotag-Hs.HRAS

Plasmid

#159681

Purpose

Mammalian protein expression of HRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.HRAS (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_GSK3B_WT

Plasmid

#82126

Purpose

Gateway Donor vector containing GSK3B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GSK3B (GSK3B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_WT

Plasmid

#82151

Purpose

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD5_WT

Plasmid

#82217

Purpose

Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD5 (SMAD5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ESR1_WT

Plasmid

#82208

Purpose

Gateway Donor vector containing ESR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ESR1 (ESR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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R713-M91-303: CMV51p> Halotag-Hs.NRAS

Plasmid

#159682

Purpose

Mammalian protein expression of NRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.NRAS (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVtight-HNF1A-FLAG-Hygro

Plasmid

#183232

Purpose

Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Use

Lentiviral

Tags

AviTag and FLAG tag

Promoter

tight TRE

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSPA5_WT

Plasmid

#82132

Purpose

Gateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSPA5 (HSPA5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_XBP1_WT

Plasmid

#82168

Purpose

Gateway Donor vector containing XBP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XBP1 (XBP1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

Plasmid

#213527

Purpose

Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

D90A and E92A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_OC43_NSP14

Plasmid

#213519

Purpose

Expresses NSP14 of hCoV OC43

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV OC43 NSP14 (ORF1ab hCoV OC43)

Tags

His-SUMO tag

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_NL63_NSP14

Plasmid

#213520

Purpose

Expresses NSP14 of hCoV NL63

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV NL63 NSP14 (ORF1ab hCoV NL63)

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_229E_NSP14

Plasmid

#213521

Purpose

Expresses NSP14 of hCoV 229E

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV 229E NSP14

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_FH_WT

Plasmid

#82210

Purpose

Gateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FH (FH Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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