We narrowed to 8,324 results for: gnal

1. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. gp64-mVEGF-C-HisTag-pENTR

   Plasmid

   #64065

   Purpose

   Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

   Insert

   vascular endothelial growth factor C (Vegfc Mouse)
   

   Use

   Tags

   His-tag

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pf113-bio

   Plasmid

   #47729

   Purpose

   Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised Pf113
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HCMVgO-8his (TB40/E)

   Plasmid

   #136453

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   envelope glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HCMVgO-8his (Merlin)

   Plasmid

   #136454

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-DIO-erHRP(N175S mutant)

   Plasmid

   #79906

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   AAV and Cre/Lox

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Promoter

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

   Plasmid

   #194461

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAMA-bio

    Plasmid

    #47747

    Purpose

    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised GAMA
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid

    #85820

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc Dam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A.

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

    Plasmid

    #194463

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PA-mCherry1-ER-5

    Plasmid

    #57174

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Article

    York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

    Insert

    ER (CALR Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. nDHFR173-gs10-NLS-VPR

    Plasmid

    #188262

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nDHFR173 (DHFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. P41-bio

    Plasmid

    #47739

    Purpose

    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P41
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZIKV_NASBA_27B

    Plasmid

    #75010

    Purpose

    Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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