We narrowed to 9,242 results for: ef1

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Lenti-EF1α-PPAT-WPRE

Plasmid

#250408

Purpose

Lentiviral expression of PPAT under EF1α core promoter.

Depositor

Stefan Kubicek

Article

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

PPAT (PPAT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1

Plasmid

#249270

Purpose

Expression of GLI1

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA + 3xGS linker

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-RPA194

Plasmid

#247348

Purpose

Expresses HaloTag-RPA194

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-fibrillarin-FRT

Plasmid

#247341

Purpose

FLP/FRT based mammalian expression vector for EGFP-fibrillarin driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Imai et al Mol Biol Cell. 2017 Nov 7;28(23):3349-33

Insert

fibrillarin (FBL) (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-coilin-FRT

Plasmid

#247448

Purpose

FLP/FRT based mammalian expression vector for EGFP-coilin, driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Itoh et al Life Sci Alliance. 2021 Feb 3;4(4):e2020

Insert

coilin (COIL) (COIL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-HaloTag-FRT

Plasmid

#247338

Purpose

Expresses H2B-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-sgRXRa4-1; EF1a-mScarlet

Plasmid

#242889

Purpose

For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

Depositor

Elaine Fuchs

Article

Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

Insert

sgRNA targeting Rxra

Use

CRISPR and Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-2-EF1a-LibVec

Plasmid

#239593

Purpose

expresses non-targeting (NT) guide#2

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec

Plasmid

#239600

Purpose

expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec

Plasmid

#239599

Purpose

expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec

Plasmid

#239598

Purpose

expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec

Plasmid

#239597

Purpose

expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-1-EF1a-LibVec

Plasmid

#239592

Purpose

expresses non-targeting (NT) guide#1

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH

Plasmid

#239716

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40

Plasmid

#239717

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE

Plasmid

#239718

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-Ef1a-SOX17WT-3xFLAG

Plasmid

#216198

Purpose

Overexpress wild-type human SOX17 in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-Ef1a-SOX17E57D-3xFLAG

Plasmid

#216199

Purpose

Overexpress human SOX17E57D in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-Ef1a-SOX17E57P-3xFLAG

Plasmid

#216200

Purpose

Overexpress human SOX17E57P in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

Plasmid

#223536

Purpose

Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

LaG16_synNotch_tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP-FMRP-bGH

Plasmid

#235265

Purpose

ComMAND EGFP-FMRP base gene

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2-bGH

Plasmid

#235275

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge