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pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMU-PDESr

Plasmid

#61206

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG-3xHA

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDest-eGFP-N1

Plasmid

#31796

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pCK122 pDESTattB_exorh:mCherry

Plasmid

#213511

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-FLAG

Plasmid

#183511

Purpose

Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Type

Empty backbone

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2M

Plasmid

#15798

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2M (UBE2M Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-DEST

Plasmid

#119747

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)

Depositor

Martin Reijns, Andrew Jackson

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Use

Tags

C-term v5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-DEST

Plasmid

#119749

Purpose

Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

Depositor

Martin Reijns, Andrew Jackson

Type

Empty backbone

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Martin Reijns, Andrew Jackson

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ150 - pDESTttTi5605[R4-R3]

Plasmid

#19329

Depositor

Erik Jorgensen

Article

Christian et al

Insert

pDESTttTi5605[R4-R3]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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