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We narrowed to 1,118 results for: cas12

Showing: 721 - 740 of 1118 results

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pUC19-U6-tRNAx2

Plasmid

#123641

Purpose

Mammalian expression, Genome editing, backbone

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-VP64-2AmScarlet

Plasmid

#121963

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64 module fused with MCP, recruited by MS2 hairpin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMB26

Plasmid

#223009

Purpose

Cas12a and Csy4 with 5' target (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' target (I)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB27

Plasmid

#223010

Purpose

Cas12a and Csy4 with 3' target (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 3' target (I)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB45

Plasmid

#223012

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB51

Plasmid

#223013

Purpose

Cas12a and Csy4 with 5' and 3' targets (II) for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (II)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB61

Plasmid

#223014

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET27b+R

Plasmid

#188245

Purpose

Empty backbone vector for protein expression in Escherichia coli and his-tag purification

Depositor

Yiping Qi

Article

Fang et al Methods Mol Biol. 2023;2653:153-171. doi

Type

Empty backbone

Use

Tags

6xHis tag and TEV site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Bs3-sgRNA (BsaI)

Plasmid

#188280

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Bs3-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFH132

Plasmid

#136547

Purpose

Level1 ZmUBIp::FnCas12a (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::FnCas12a (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFH133

Plasmid

#136548

Purpose

Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::LbCas12a (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFH60

Plasmid

#128186

Purpose

Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::FnCas12a (rice codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH61

Plasmid

#128187

Purpose

Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::LbCas12a (rice codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z31

Plasmid

#120074

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gCH128 (non-targeting crRNA)

Plasmid

#217339

Purpose

Non-targeting crRNA

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crNT-3

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti

Plasmid

#115797

Purpose

Expresses mTagBFP in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mTagBFP

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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