We narrowed to 15,043 results for: nol

1. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-and1 in situ probe

   Plasmid

   #52102

   Purpose

   actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

   Depositor

   Didier Stainier

   Article

   Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

   Insert

   actinodin 1 in situ probe (and1 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pES.HSV2.3a

   Plasmid

   #69593

   Purpose

   TCR a chain construct for generation of gBT-I mice

   Depositor

   Francis R. Carbone

   Article

   Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

   Insert

   TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H-2Kb

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

4. CRISPR_TRBC

   Plasmid

   #164994

   Purpose

   Expression of gRNA targeting TCRbeta constant locus

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against TRBC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. VYAIFIFQL_epitope

   Plasmid

   #165018

   Purpose

   Expression of CMV US20 (29-37) fused to IL-2 signal sequence

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV US20 (29-37)
   

   Use

   Lentiviral

   Tags

   human IL2 signal sequence at N-term

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYCDuet_D27NDHFR_WTTS

   Plasmid

   #91244

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D27N

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXJ40 GADD34 (214-513aa)

   Plasmid

   #78095

   Purpose

   mammalian expression of GADD34 214-513 aa

   Depositor

   Shirish Shenolikar

   Article

   Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

   Insert

   GADD34 (PPP1R15A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   expresses aa 214-513

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFUW-tetO-Nfat1

    Plasmid

    #139830

    Purpose

    doxycycline-inducible expression of mouse Nfat1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Nfat1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTJK517

    Plasmid

    #121474

    Purpose

    CEN TRP1 CNA1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNA1
    

    Use

    Yeast cen vector

    Tags

    Expression

    Mutation

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FB312

    Plasmid

    #203569

    Purpose

    TU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.

    Depositor

    Jose Marcos

    Article

    Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64

    Insert

    PgpdA:Nluc:Ttub
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR3_BC2

    Plasmid

    #191857

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR3_BC6

    Plasmid

    #191861

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR3_BC5

    Plasmid

    #191860

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPR3_BC4

    Plasmid

    #191859

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSNRW-Z3-Nluc

    Plasmid

    #174486

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z3-Nluc
    

    Use

    Tags

    6x Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRISPR_HLA_DPB

    Plasmid

    #164989

    Purpose

    Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPB1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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