We narrowed to 4,636 results for: pcr

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BGGLO QPCR PLASMID

Plasmid

#79905

Purpose

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

Depositor

Jeffery Miller

Article

Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

Insert

Human partial HBG and HBB gene

Promoter

No promoter

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin E

Plasmid

#68819

Purpose

Flag tagged mammalian expression of E domain

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

internal EcoRI site has silent mutation

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCR4-MBII52-probe

Plasmid

#67647

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII52 snoRNA (Snord115 Mouse)

Use

For in vitro transcription

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-M26I

Plasmid

#29357

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Mutation

Met26Ile

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pFA6a-TRP1-PGAL1

Plasmid

#41606

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

TRP1 reverse orientation

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt-3HA

Plasmid

#39284

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Article

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Mutation

n/a

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-TRP1

Plasmid

#26874

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-FLPo-3xP3-RFP

Plasmid

#127561

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

FLPo

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-QF2-3xP3-RFP

Plasmid

#127559

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

QF2

Use

CRISPR

Expression

Insect

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCru5-/CGA-mEGFP-IRES-mCherry

Plasmid

#49224

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

Viral LTR (or CMV)

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::mCherry-H11

Plasmid

#211940

Purpose

dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

Depositor

Evgeny Kvon

Article

Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

HSP68

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Sgo1 N61I

Plasmid

#108496

Purpose

expression of vsv-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-1.1-2.01 beta

Plasmid

#69591

Purpose

OT-II TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR beta clone 1.1 (Tcrb Mouse)

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4a-ISH-2kb

Plasmid

#68029

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4A (Sema4a Mouse)

Use

Mrna in situ hybridization probe

Mutation

a 2kb cDNA fragment for ISH probe synthesis

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCR4-Dact1(1250-1601)

Plasmid

#52046

Purpose

To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

Depositor

Ben Cheyette

Article

Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

Insert

Dact1(1250-1601) (Dact1 Mouse)

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
(G38) pCRII-Wnt10b/b-globin

Plasmid

#25877

Depositor

Ormond MacDougald

Article

Longo et al J Biol Chem. 2004 Aug 20. 279(34):35503-

Insert

Wnt10b / b-globin (Wnt10b Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-HBH

Plasmid

#26885

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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