Addgene

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hsa-miR-100 plix4.03

Plasmid

#121128

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-100 (MIR100 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSCALPS_miR-146aSponge_mCherry_GFP

Plasmid

#149718

Purpose

Lentiviral vector expressing a miR-146a decoy sponge

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

14X miR-146a binding sites

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G to T mutation in position 75 of the insert

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3(R982A)

Plasmid

#39487

Depositor

Sonja Vermeren

Article

Krugmann et al Curr Biol. 2004 Aug 10;14(15):1380-4.

Insert

ARAP3(R982A) (ARAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R982A = Rho GAP mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pER8 cHA-AIP2

Plasmid

#118687

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AIP2 (AT5G20910 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

LexA

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DKAR-Nuc

Plasmid

#45499

Depositor

Alexandra Newton

Article

Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

Insert

DKAR-Nuc

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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vector plix 4.03

Plasmid

#121130

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 146a-Dendra2

Plasmid

#97113

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 146a Dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 199-3-Dendra2

Plasmid

#97119

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 199 3 Dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3(R1155E)

Plasmid

#39488

Depositor

Sonja Vermeren

Article

Krugmann et al Curr Biol. 2004 Aug 10;14(15):1380-4.

Insert

ARAP3(R1155E) (ARAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R1155E = Ras binding domain mutation

Promoter

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pHL 1454

Plasmid

#53019

Purpose

AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

fimB

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Mutation

Promoter

pLlac0-1

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1916

Plasmid

#53023

Purpose

AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

fimB

Use

Synthetic Biology

Tags

Asp terminator and RBS(st7)

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

Plasmid

#216664

Purpose

Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

ETV2 (Etv2 Mouse)
BFP
HygromycinR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015910

Plasmid

#226287

Purpose

Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Isoeugenol synthase 1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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