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pcDNA 3.1(-) rat C/EBP beta (LIP)

Plasmid

#12562

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pTHAP12-N1.1.2-gDNA

Plasmid

#175860

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

THAP12-Nickase2

Use

CRISPR

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCHCHD3-N2.1.2-gDNA

Plasmid

#175858

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CHCHD3-Nickase2

Use

CRISPR

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCHCHD3-N1.1.1-gDNA

Plasmid

#175857

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CHCHD3-Nickase1

Use

CRISPR

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pE4F1.1.0-gDNA

Plasmid

#132451

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

E4F1 (E4F1 Human)

Use

CRISPR

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP beta (LAP)

Plasmid

#12557

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97 IRES EGFP

Plasmid

#130707

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-LC122-TAG

Plasmid

#226839

Purpose

Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 122 of light chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-HC121-TAG

Plasmid

#226836

Purpose

Contains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 121 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir44-4

Plasmid

#46707

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-4 (mir-44 Synthetic, Nematode)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pTOX.1.0-gDNA

Plasmid

#132470

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

TOX (TOX Human)

Use

CRISPR

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRHOXF2B.1.0-gDNA

Plasmid

#132462

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

RHOXF2B (RHOXF2B Human)

Use

CRISPR

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZNF780A.1.0-gDNA

Plasmid

#132446

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

ZNF780A (ZNF780A Human)

Use

CRISPR

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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