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non-targeting control gRNA (BRDN0001146085)

Plasmid

#80216

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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V207 pRETRO-Triple-Flag SD - Acceptor

Plasmid

#11709

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox and Retroviral; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
beta4-nAChR (DPM negative control)

Plasmid

#86651

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (control)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149294)

Plasmid

#80192

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147095)

Plasmid

#80222

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001162447)

Plasmid

#80239

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146327)

Plasmid

#80188

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-aSYNUCLEIN WT

Plasmid

#247940

Purpose

AAV transfer plasmid encoding the human α-SYN under the control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Oliveras-Salva et al Mol Neurodegener. 2013 Nov 25;8:44. doi:

Insert

alpha synuclein WT (SNCA Human)

Use

AAV

Promoter

CMVenh synapsin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shControl-mCherry-CAAX

Plasmid

#227689

Purpose

Membrane tethered mCherry and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSA358_pAAV-SCP1-Intron-eGFP-CS1

Plasmid

#215513

Purpose

Single stranded AAV eGFP reporter vector with SCP1 promoter.

Depositor

Stein Aerts

Article

Hecker et al Science. 2025 Jan 2;387(6735):eadp3957.

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

SCP1

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

Plasmid

#239742

Purpose

Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

Plasmid

#239743

Purpose

Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-ER WPRE

Plasmid

#236230

Purpose

AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 P278D P281D

Plasmid

#221320

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P278D P281D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT025-24 Angstrom 12TMD de novo_mRFP1_3x FLAG

Plasmid

#202507

Purpose

Express 24 Angstrom 12TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

24 Angstrom 12TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT026-32 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202508

Purpose

Express 32 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

32 Angstrom 4TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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