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We narrowed to 7,730 results for: Pol

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Showing: 7381 - 7400 of 7730 results

  1. pCJ1023

    Plasmid
    #228763
    Purpose
    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228763-plasmid-map-sequence-id-455783

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  3. 8V04

    Plasmid
    #220879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH and Honeybee melittin signal
    Mutation
    S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220879-plasmid-map-sequence-id-436702

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  6. pFBOH-MHL_LRRK2:2141-2527

    Plasmid
    #210899
    Purpose
    Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    LRRK2:T2141-E2527 (LRRK2 Human)
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    M2397T natural variant ( VAR_024965)
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    210899-plasmid-map-sequence-id-426130

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  7. pFBD-BirA_ATG16L1:303-607

    Plasmid
    #210942
    Purpose
    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    ATG16L1:G303-Y607 (ATG16L1 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210942-plasmid-map-sequence-id-424570

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  8. pFBD-BirA_CDC40:236-578

    Plasmid
    #210885
    Purpose
    Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    CDC40:E236-W578 (CDC40 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210885-plasmid-map-sequence-id-424501

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  9. pLX304-EZH1-Y727F

    Plasmid
    #203582
    Purpose
    Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Publication
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    changed Tyrosine 727 to Phenylalanine for partialā€¦
    Promoter
    CMV
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203582-plasmid-map-sequence-id-423636

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  10. pLX304-EZH1-dPSET

    Plasmid
    #203586
    Purpose
    Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Publication
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    deletion of aa 744-747 and partial resistance to ā€¦
    Promoter
    CMV
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203586-plasmid-map-sequence-id-423597

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  18. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

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  20. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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Showing: 7381 - 7400 of 7730 results