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pCDNA5/FRT/TO_COX8-Halo-PanK

Plasmid

#197949

Purpose

Expression of FRET_control in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CLK2

Plasmid

#155459

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK2 (CLK2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BTG1

Plasmid

#155425

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG1 (BTG1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Article

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX301-RFP-GGA3 NGAT

Plasmid

#162031

Purpose

Expresses NGAT sequence of GGA3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

GGA3 NGAT (GGA3 Human)

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-TALE-mCherry-15b

Plasmid

#138287

Purpose

Express tTALE-mCherry

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-mCherry

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2(delta2-6)-mCherry

Plasmid

#104182

Purpose

Expresses fusion of CRY2(delta aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(delta2-6)-mCherry

Expression

Mammalian

Mutation

CRY2PHR with deletion of aa 2-6

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(L91P)

Plasmid

#99726

Purpose

Mammalian expression of Flag-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

L91P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(K84T)

Plasmid

#99727

Purpose

Mammalian expression of Flag-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

K84T

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.17.1

Plasmid

#98189

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.23.1

Plasmid

#98206

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.2

Plasmid

#98200

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-005

Plasmid

#78692

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Neurod1

Plasmid

#74396

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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J23100_AB

Plasmid

#65980

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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