We narrowed to 9,264 results for: pho;

1. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

   Plasmid

   #192445

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

   Plasmid

   #22023

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450A.Rac1 s…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

3. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter;

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse, Human)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-N-HA-Gp78

   Plasmid

   #123591

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   autocrine motility factor receptor (Amfr Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-OptoFGFR1-Y766F

   Plasmid

   #59777

   Purpose

   Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-HNRNPD_sgRNA1

   Plasmid

   #201598

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HNRNPD (HNRNPD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M

   Plasmid

   #102592

   Purpose

   Lentivector to express STING V155M HAQ and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M R71H-G230A-R293Q

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

   Plasmid

   #36881

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-N-3XFLAG-Gp78

    Plasmid

    #123590

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. LENTICRISPR-PABPC1_sgRNA1

    Plasmid

    #201608

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29-hMIGA2(306-593)-6xhis

    Plasmid

    #192837

    Purpose

    Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1A-PIK3CA E545K

    Plasmid

    #180032

    Purpose

    Entry vector of PIK3CA E545K for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid

    #163340

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p53 S23A targeting vector

    Plasmid

    #12167

    Depositor

    Tyler Jacks

    Article

    MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Ser23 mutation (S23A) in exon 2 and puromycin gen…

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA6-C-3XFLAG-m-Lmbr1l

    Plasmid

    #123593

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    limb region 1 like (Lmbr1l Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-N-HA-m-Lmbr1l

    Plasmid

    #123594

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    limb region 1 like (Lmbr1l Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge