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pAtCas9-dead_3

Plasmid

#91167

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMT1121

Plasmid

#8620

Depositor

Robert Hartley

Publication

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa, barstar

Expression

Bacterial

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-CyTERM-mTagBFP2-24xMS2

Plasmid

#254843

Purpose

an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of MS2 RNA tag

Depositor

Jiahui (Chris) Wu

Publication

Pham et al Nat Methods. 2026 Jan;23(1):165-174. doi

Insert

CyTERM-mTagBFP2-24xMS2

Expression

Bacterial

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239587

Purpose

Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

Depositor

Andreas Moor

Publication

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Type

Empty backbone

Use

Sleeping beauty transposone

Expression

Mammalian

Promoter

hU6

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA360 - pBA904 Puro-T2A-GFP

Plasmid

#238164

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

Lentiviral

Tags

GFP

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUSE-URA3-Sec7-msYFP2

Plasmid

#218969

Purpose

Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

Depositor

Benjamin Glick

Insert

SEC7-msYFP2 (SEC7 Budding Yeast)

Tags

msYFP2

Expression

Yeast

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Publication

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Publication

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Publication

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CLK2

Plasmid

#155459

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK2 (CLK2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BTG1

Plasmid

#155425

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG1 (BTG1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Publication

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX301-RFP-GGA3 NGAT

Plasmid

#162031

Purpose

Expresses NGAT sequence of GGA3

Depositor

David Bryant

Publication

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

GGA3 NGAT (GGA3 Human)

Use

Lentiviral

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-TALE-mCherry-15b

Plasmid

#138287

Purpose

Express tTALE-mCherry

Depositor

Kyubong Jo

Publication

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-mCherry

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Publication

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Publication

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2(delta2-6)-mCherry

Plasmid

#104182

Purpose

Expresses fusion of CRY2(delta aa 2-6) with mCherry

Depositor

Bianxiao Cui

Publication

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(delta2-6)-mCherry

Expression

Mammalian

Mutation

CRY2PHR with deletion of aa 2-6

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.23.1

Plasmid

#98206

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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