We narrowed to 14,009 results for: flag

1. pEBTetBl-CLIP-Cep78

   Plasmid

   #136876

   Purpose

   Mammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-Cep78 (CEP78 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GPR144-Tango

   Plasmid

   #66321

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR144 (ADGRD2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CBR1_pLENTI-CAG-IRES-GFP

   Plasmid

   #177008

   Purpose

   Mammalian lentiviral expression vector encoding CBR1

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   CBR1 (CBR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-ULK2-K39I

   Plasmid

   #97076

   Purpose

   Expresses mutant ULK2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

   Insert

   ULK2 (ULK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 39 to Isoleucine

   Promoter

   CMV

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiex1-SYNAC

   Plasmid

   #82710

   Purpose

   Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

   Insert

   SYNAC (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET23a_mod_RBPMS_FL

   Plasmid

   #59393

   Purpose

   Plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   6xHis, FLAG, and HA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEBTet-SNAP-Cep27

   Plasmid

   #136808

   Purpose

   Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep27 (HAUS2 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAD108_Arg273His_pCMV6Entry-hIRG1

   Plasmid

   #124878

   Purpose

   Expresses hCAD Arg273His mutant in mammalian cells

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   myc and FLAG tags

   Expression

   Mammalian

   Mutation

   mutated Arg273 to His

   Promoter

   CMV

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 33-G-X1-pEF1-puro

   Plasmid

   #111604

   Purpose

   Express human 33-G-X1 in 293T cells

   Depositor

   Timothy Springer

   Article

   Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

   Insert

   human LRRC33 and GARP chimeria (NRROS Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPR21-Tango

    Plasmid

    #66345

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR21 (GPR21 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GPR157-Tango

    Plasmid

    #66331

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR157 (GPR157 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-HF1-PGK-Puro

    Plasmid

    #110857

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HOXA10 (human) bluescript

    Plasmid

    #8617

    Depositor

    Corey Largman

    Article

    Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

    Insert

    HOXA10 (HOXA10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    improved Ribosomal binding site (NcoI site) at AT…

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pECE M2-SH3PXD2A 8 serines mutated to alanines

    Plasmid

    #69816

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23a_mod_RBPMS2_FL

    Plasmid

    #59394

    Purpose

    Plasmid for bacterial overexpression of RBPMS2

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Use

    Tags

    6xHis, FLAG, HA, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RF-118-UB-BRCA1

    Plasmid

    #78960

    Purpose

    Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Article

    Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-1155

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-ADAM7 (wt)

    Plasmid

    #31139

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM7 (ADAM7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEBTetBl-CLIP-Cep70

    Plasmid

    #136870

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBTetDBl-CLIP-Cep70

    Plasmid

    #136871

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5 N539

    Plasmid

    #25972

    Depositor

    Rob Lewis

    Article

    Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted amino acids 540-873

    Promoter

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pEBTetBl-CLIP-Cep41

    Plasmid

    #136843

    Purpose

    Mammalian expression of the centrosomal protein Cep41 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep41 (CEP41 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEN_TTmiRC2_p53DD

    Plasmid

    #136520

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    This construct was created by PCR from the pBabe …

    Promoter

    Available Since

    Oct. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEBTetDBl-CLIP-Cep97

    Plasmid

    #136850

    Purpose

    Mammalian expression of the centrosomal protein Cep97 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep97 (CEP97 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEBTetD-SNAP-Cep97

    Plasmid

    #136801

    Purpose

    Mammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep97 (CEP97 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pECE M2-SH3PXD2A 6 serines mutated to alanines

    Plasmid

    #69815

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pECE M2-SH3PXD2A S974A

    Plasmid

    #69814

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV5 C540

    Plasmid

    #25971

    Depositor

    Rob Lewis

    Article

    Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletes amino acids 1-539

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

28. myc-Med4 pBacPAK8

    Plasmid

    #15364

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med4 (MED4 Human, N/A)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pLenti-VQR-P2A-Puro

    Plasmid

    #110848

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-BE3-FNLS

    Plasmid

    #110836

    Purpose

    CMV expression vector for BE3-FNLS construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge