We narrowed to 12,359 results for: NSI

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pDEST40-2XHA-Src-S4-UD

Plasmid

#140304

Purpose

Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-KD

Plasmid

#140296

Purpose

Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD-KD

Plasmid

#140300

Purpose

Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

lacks the SH3 and SH2 domains

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp

Plasmid

#108481

Purpose

inducible expression of vsv-CenpB-1-166-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(2Q)

Plasmid

#21752

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
TTR C10S S100C

Plasmid

#190010

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S100C

Promoter

t7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm2

Plasmid

#20879

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Predicted miRNA binding site 2 (Position ~1100) G…

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only
pQC hKGA.wt V5-His IRES G418

Plasmid

#110390

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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DPYS-FLAG

Plasmid

#61618

Purpose

To study the effect of DPYS activity on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidinase (DPYS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3b(1454/3172)P2P-wt

Plasmid

#21404

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
BRCA1-(1316-1538)

Plasmid

#35079

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1316-1538

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Promoter

PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF16-1/1

Plasmid

#91334

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF16-1/1 (ARHGEF16 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF26-1/1

Plasmid

#91342

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF26-1/1 (ARHGEF26 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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BRCA1-(498-663)

Plasmid

#35070

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 498-663

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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