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pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pOX-twk18

Plasmid

#16430

Depositor

Larry Salkoff

Article

Kunkel et al J Neurosci. 2000 Oct 15. 20(20):7517-24.

Insert

Tandem pore domain K+ channel twk-18 (twk-18 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pETO18G-H7C/R86C

Plasmid

#27403

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX8 and Thrombin

Expression

Bacterial

Mutation

Changes His 7 to Cys and Arg 86 to Cys

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTU-B-005

Plasmid

#124413

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-005

Plasmid

#124414

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBScMXT + egl2

Plasmid

#16209

Depositor

Larry Salkoff

Article

Weinshenker et al J Neurosci. 1999 Nov 15. 19(22):9831-40.

Insert

K+-channel KCNQ (egl-2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pQO18 TEV-L400C

Plasmid

#27400

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX10 and TEV

Expression

Bacterial

Mutation

Changed Leu 400 to Cys

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4a-hFJX1-Flag

Plasmid

#46931

Purpose

expression of human FJX1-FLAG in mammalian cells

Depositor

R.D. Beauchamp, Natasha Deane

Insert

FJX1 (FJX1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pFUW mTagBFP2

Plasmid

#239562

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJEC619

Plasmid

#182955

Purpose

SYNZIP18-Cascade.

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Insert

SynZip18-Cascade

Tags

SynZip18

Expression

Bacterial

Mutation

SynZip18 fusion to CasA

Promoter

J23150

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDUP

Plasmid

#223515

Purpose

cogfp under control of Ptac

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Insert

CoGFP

Tags

His tag

Expression

Bacterial

Promoter

Ptac and Ptac and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAND-MCS

Plasmid

#223514

Purpose

Plasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Type

Empty backbone

Expression

Bacterial

Promoter

Ptac and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP41

Plasmid

#212874

Purpose

Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP31

Plasmid

#212864

Purpose

Expresses the Saccharomonospora piscinae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP44

Plasmid

#212877

Purpose

Expresses the Saccharopolyspora shandongensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP35

Plasmid

#212868

Purpose

Expresses the Saccharopolyspora antimicrobica LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP36

Plasmid

#212869

Purpose

Expresses the Saccharopolyspora elongata LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSR1054

Plasmid

#208740

Purpose

Characterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PAmy

Use

Synthetic Biology

Expression

Bacterial

Promoter

PAmy

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1055

Plasmid

#208741

Purpose

Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHrcA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PHrcA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03

Plasmid

#188556

Purpose

Binary vector with MpIGPDm as a selective marker in Marchantia polymorpha (igpd mutants)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

MpIGPDm

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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