We narrowed to 16,006 results for: grna

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pX459-HypaCas9-ECT2_sgRNA

Plasmid

#183873

Purpose

pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9336 (pgRNA_II-1_NatMX)

Plasmid

#161589

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

Depositor

Irina Borodina

Publication

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA-XMAS-2x

Plasmid

#164414

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Publication

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pU6 SgRNA GAPDH

Plasmid

#162736

Purpose

Mammalian expression

Depositor

Amit Choudhary

Publication

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Spacer sequence for GAPDH

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Publication

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2842C

Plasmid

#139324

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

Depositor

Alberto Ciccia

Publication

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA to insert BRCA2 R2842C using base editing

Expression

Mammalian

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Publication

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA to insert BRCA1 E638K using base editing

Expression

Mammalian

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 V572I

Plasmid

#139321

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

Depositor

Alberto Ciccia

Publication

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA to insert BRCA2 V572I using base editing

Expression

Mammalian

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pH-STEME-NG-esgRNA

Plasmid

#138136

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS

Expression

Plant

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Publication

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA

Use

CRISPR

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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U6_sgRNA_CAG_hSt1Cas9_LMD9

Plasmid

#110626

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Publication

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Publication

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Promoter

hsyn

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH-PABE-7-sgRNA

Plasmid

#115621

Purpose

Targeted A to G in rice

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pegRNA2_tevopreQ1

Plasmid

#212039

Purpose

Bacterial expression of the pegRNA for genome editing in E. coli

Depositor

Quanjiang Ji

Publication

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

pegRNA

Use

CRISPR

Promoter

J23119

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCnCas12f1HS_sgRNA_MS13_empty

Plasmid

#204999

Purpose

CnCas12f1 with MS13 sgRNA site for Human cell genome editing

Depositor

Quanjiang Ji

Publication

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Type

Empty backbone

Use

CRISPR

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

Plasmid

#110624

Purpose

A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

SV40 NLS

Expression

Mammalian

Promoter

LP1B and hU6

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAT159_AAV_p3_PE7-C_hU6_epegRNA

Plasmid

#251105

Purpose

Encodes the C terminal of the PE7 prime editor in an AAV expression plasmid + pegRNA cloning casette

Depositor

Gerald Schwank

Publication

Talas et al Sci Transl Med. 2026 May 13;18(849):eaec

Insert

PE7 C-terminal

Use

AAV and CRISPR

Promoter

p3

Available since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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