We narrowed to 32,145 results for: eng

1. pCDNA3.3TOPO-T7-hCas9

   Plasmid

   #161876

   Purpose

   In vitro transcription of SpCas9

   Depositor

   Stephane Pelletier

   Article

   Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

   Insert

   human codon optimized SpCas9
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPTn2P-PTK

   Plasmid

   #85604

   Purpose

   Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

   Depositor

   Karl Clark, Scott Fahrenkrug

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Passport transposon with a puromycin-thymidine kinase fusion
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMYT013

   Plasmid

   #180666

   Purpose

   Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

   Depositor

   Tom Ellis

   Article

   Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

   Insert

   pTet5
   

   Use

   Synthetic Biology

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO 9.3_bCas9-CP230

   Plasmid

   #121497

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH124

   Plasmid

   #102951

   Purpose

   Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

   Depositor

   Peter Espenshade

   Article

   Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

   Insert

   APEX2-NES
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_EF1a-->GFPlig

   Plasmid

   #162223

   Purpose

   Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ146

   Plasmid

   #69298

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GLI2 his

   Plasmid

   #87097

   Purpose

   expresses GLI2 in mammalian cells

   Depositor

   Martin Fernandez-Zapico

   Insert

   GLI2 (GLI2 Human)
   

   Tags

   6x His, T7 tag, and Xpress tag

   Expression

   Mammalian

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMEV4

   Plasmid

   #110539

   Purpose

   The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

   Depositor

   William B. Whitman

   Article

   Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. p361_DG

    Plasmid

    #234683

    Purpose

    Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Tags

    His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Expression

    Bacterial

    Mutation

    No

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. lacI-PT7-gusA-pBAV1k

    Plasmid

    #30504

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucoronidase (gusA Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCJ162

    Plasmid

    #175055

    Purpose

    lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

    Depositor

    David Nielsen

    Article

    Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

    Insert

    lox66::gmR::lox71
    

    Use

    Unspecified

    Promoter

    n/a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV.IRES.hCD4

    Plasmid

    #35712

    Depositor

    Patricia Ernst

    Article

    Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    IRES
    hCD4
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

14. FBE/SBE-Luc

    Plasmid

    #16525

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FBE/SBE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pXP220

    Plasmid

    #26832

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXP218

    Plasmid

    #26831

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEV-G1-Ph

    Plasmid

    #46814

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Promoter

    TEF1 and PGK1

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEV-G2-Km

    Plasmid

    #46815

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Promoter

    TEF1 and PGK1

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHK352.3

    Plasmid

    #235473

    Purpose

    Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    sgRNA3
    

    Use

    Synthetic Biology

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_CMV_Melt-30-mCherry

    Plasmid

    #234061

    Purpose

    Encodes a Melt variant with a switching temperature of 30°C

    Depositor

    Lukasz Bugaj

    Article

    Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

    Insert

    Melt-30-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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