We narrowed to 17,805 results for: Met;

1. pcDNA3-mNeptune2.5

   Plasmid

   #51310

   Purpose

   Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2.5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCardinal-Lysosomes-20

   Plasmid

   #56166

   Purpose

   Localization: Lysosome Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   LAMP1
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pEM040 [Multiome Perturb-seq sgRNA expression vector]

   Plasmid

   #224898

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

   Depositor

   Thomas Norman

   Article

   Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-P-CAG-GFPluc2

   Plasmid

   #80493

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   GFPluc2
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

   Plasmid

   #189755

   Purpose

   Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mTurquoise2_spatial-linker-P2A_mScarlet-I3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEx-4T-1-YTHDF1

   Plasmid

   #70087

   Purpose

   Encode YTHDF1 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. modRNAc0-ABE8e-P2A-GFP

   Plasmid

   #178177

   Purpose

   IVT template plasmid for making ABE8e-P2A-GFP modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   ABE8e
   

   Use

   Ivt template plasmid

   Tags

   P2A-GFP

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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