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Plasmid#183942PurposeExpresses catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli )
UseTagsExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK649
Plasmid#72399PurposeProduces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)DepositorInsertamidophosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf
Plasmid#65825Purposezwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertzwf guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA
Plasmid#65818PurposeudhA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2
Plasmid#65817PurposegltA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA2 guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1914
Plasmid#53042PurposePconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and hfqleadExpressionBacterialMutationPromoterPconNoHindM2 and pLlacO-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBI
Plasmid#17816DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
PAS528 (TJF059)
Plasmid#65602PurposepETDuet-1 Containing FadD-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationPromoterT7 lacOAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
UseTagsExpressionBacterialMutationPromoterP-tetAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only