Addgene

We narrowed to 1,323 results for: GPCR

Showing: 371 - 380 of 1323 results

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NbV5-TEV219S153N

Plasmid

#201488

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-TEV219S153N

Use

Tags

TEV219 S153N

Expression

Mammalian

Mutation

S153N in TEV219

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-luc2

Plasmid

#194384

Purpose

Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

6xCRE-CMVmin-luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV minimal

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-MLPmin-luc2

Plasmid

#194383

Purpose

Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

10xUAS-luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

MLP minimal

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

Plasmid

#230022

Purpose

Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230020

Purpose

AAV vector expressing anti-mCherry PAGER(Gq) in neurons

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM4D

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-mIFNγ

Plasmid

#230004

Purpose

Gal4-dependent expression of secreted mouse IFNγ

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-mIFNγ (Ifng Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKART391A

Plasmid

#60446

Purpose

Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKART391A

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

changed Threonine 391 to Ananine

Promoter

CAG

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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