Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 849 results for: mRuby

Clear keywords
Showing: 741 - 760 of 849 results

  2. pOCC291

    Plasmid
    #118867
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mRuby3
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118867-plasmid-map-sequence-id-232275

    Enlarge

  3. pUC57-Amp_CRISPIE donor B2

    Plasmid
    #172843
    Purpose
    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-0) encoding mRuby3
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172843-plasmid-map-sequence-id-340940

    Enlarge

  4. pODN-mR2-ACTB

    Plasmid
    #183868
    Purpose
    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB homology arms with mRuby2-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183868-plasmid-map-sequence-id-362451

    Enlarge

  5. pMIA3-Pansio

    Plasmid
    #214445
    Purpose
    Expresses eSpCas9 and gRNA targeting Pansio-1 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214445-plasmid-map-sequence-id-433843

    Enlarge

  7. pCAG-postASAP-p2a-ChrimsonR

    Plasmid
    #178794
    Purpose
    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR
    Depositor
    Rafael Yuste
    Publication
    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FingR.PSD95, ASAP2f and ChrimsonR
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    mutations in amino acids L146G, S147T N149R, S150…
    Promoter
    CAG
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178794-plasmid-map-sequence-id-350048

    Enlarge

  8. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Publication
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109399-plasmid-map-sequence-id-211963

    Enlarge

  9. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid
    #181857
    Purpose
    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FluoSTEP-JNKAR
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181857-plasmid-map-sequence-id-363201

    Enlarge

  10. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid
    #181856
    Purpose
    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FluoSTEP-AktAR
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181856-plasmid-map-sequence-id-362780

    Enlarge

  11. pMIA3-Keppel

    Plasmid
    #214446
    Purpose
    Expresses eSpCas9 and gRNA targeting Keppel-19 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214446-plasmid-map-sequence-id-431687

    Enlarge

  13. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

    Enlarge

  14. pMM0641

    Plasmid
    #129006
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Publication
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129006-plasmid-map-sequence-id-259445

    Enlarge

  15. pMIA3-Olônne

    Plasmid
    #214447
    Purpose
    Expresses eSpCas9 and gRNA targeting Olônne-18 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214447-plasmid-map-sequence-id-431691

    Enlarge

  18. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Publication
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Expression
    Yeast
    Promoter
    TEF1
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

    Enlarge

  19. pMM2_4

    Plasmid
    #183057
    Purpose
    HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Publication
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mRuby2
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mRuby2 and mTurquoise2
    Expression
    Yeast
    Promoter
    HAA1 and YGP1
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183057-plasmid-map-sequence-id-363440

    Enlarge

  20. ARB365

    Plasmid
    #124048
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124048-plasmid-map-sequence-id-252519

    Enlarge

Showing: 741 - 760 of 849 results