We narrowed to 1,621 results for: nif

1. pOpen-Bst HF

   Plasmid

   #165539

   Purpose

   High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Bst HF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pOpen-9N7lig

   Plasmid

   #165514

   Purpose

   9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C).

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   9 Degrees North DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pOpen-T4-BGT

   Plasmid

   #165530

   Purpose

   T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   T4-BGT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pOpen-Eco31I

   Plasmid

   #165506

   Purpose

   Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Eco31I
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOpen-9N7polA

   Plasmid

   #165500

   Purpose

   Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   9 degrees N-7 DNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOpen-THEAQlig

   Plasmid

   #165535

   Purpose

   Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Taq DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pOpen-UDG

   Plasmid

   #165531

   Purpose

   Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   UDG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pOpen-Bstpol

   Plasmid

   #165543

   Purpose

   Full length DNA polymerase from Bacillus stearothermophilus.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Bst DNA Polymerase, Full Length
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pOpen-TthpolA

   Plasmid

   #165533

   Purpose

   DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Tth DNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pWPI-myogenin

    Plasmid

    #79073

    Purpose

    Lentiviral plasmid that constitutively expresses human myogenin

    Depositor

    Jennifer Lei

    Insert

    human myogenin (MYOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOpen-T4 PGD

    Plasmid

    #165550

    Purpose

    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T4 PGD (T4 Endonuclease V)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOpen-SP6rpol

    Plasmid

    #165516

    Purpose

    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    SP6 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pOpen-TEV protease

    Plasmid

    #165509

    Purpose

    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    TEV protease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pOpen-DpnIR

    Plasmid

    #165529

    Purpose

    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    DpnI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pOpen-PstIM

    Plasmid

    #165536

    Purpose

    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    M.PstI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pOpen-NotIR (GC)

    Plasmid

    #165507

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    NotI (GC-rich recognition site)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pOpen-ALPI

    Plasmid

    #165555

    Purpose

    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    CIP (calf intestinal phosphatase)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pOpen-T7EI

    Plasmid

    #165540

    Purpose

    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T7EI (T7 Endonuclease I)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pOpen-9N7polA (CT)

    Plasmid

    #165502

    Purpose

    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    9 degrees N-7 DNA Polymerase chain terminating
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pOpen-EcoRIM

    Plasmid

    #165515

    Purpose

    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    M.EcoRI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge